| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNA polymerase alpha catalytic subunit (81-130 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLA1 |
| Gene ID: |
5422 |
| Uniprot ID: |
DPOLA_HUMAN |
| Immunogen Region: |
81-130 aa |
| Specificity: |
DNA pol Alpha Polyclonal Antibody detects endogenous levels of DNA pol Alpha protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human DNA Polymerase alpha at the amino acid range 81-130 |
| Function | Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses. |
| Protein Name | Dna Polymerase Alpha Catalytic SubunitDna Polymerase Alpha Catalytic Subunit P180 |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-69205Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusCytoplasmCytosolIn The CytosolColocalizes With Rna:dna Hybrids With A Speckled Pattern |
| Alternative Antibody Names | Anti-Dna Polymerase Alpha Catalytic Subunit antibodyAnti-Dna Polymerase Alpha Catalytic Subunit P180 antibodyAnti-POLA1 antibodyAnti-POLA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
