| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4200RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PNKP |
| Gene ID: |
11284 |
| Uniprot ID: |
PNKP_HUMAN |
| Immunogen Region: |
400-500 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 400-500 of Human PNKP (NP_009185.2). |
| Immunogen Sequence: |
GSWQRCVTTCETALKQGKRV AIDNTNPDAASRARYVQCAR AAGVPCRCFLFTATLEQARH NNRFREMTDSSHIPVSDMVM YGYRKQFEAPTLAEGFSAIL E |
| Tissue Specificity | Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver. |
| Function | Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. |
| Protein Name | Bifunctional Polynucleotide Phosphatase/KinaseDna 5'-Kinase/3'-PhosphatasePolynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase |
| Database Links | Reactome: R-HSA-5649702 |
| Cellular Localisation | NucleusChromosomeLocalizes To Site Of Double-Strand Breaks |
| Alternative Antibody Names | Anti-Bifunctional Polynucleotide Phosphatase/Kinase antibodyAnti-Dna 5'-Kinase/3'-Phosphatase antibodyAnti-Polynucleotide Kinase-3'-Phosphatase Includes - Polynucleotide 3'-Phosphatase antibodyAnti-2'(3'-Polynucleotidase - Polynucleotide 5'-Hydroxyl-Kinase antibodyAnti-PNKP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunohistochemistry analysis of CRMP2/DPYSL2 in paraffin-embedded mouse brain using [KO Validated] CRMP2/CRMP2/DPYSL2 Rabbit polyclonal antibody (STJ116781) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/95755/377906/STJ116781_1__52305.1713145321.jpg?c=1 "Immunohistochemistry analysis of CRMP2/DPYSL2 in paraffin-embedded mouse brain using [KO Validated] CRMP2/CRMP2/DPYSL2 Rabbit polyclonal antibody (STJ116781) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
