• Immunohistochemistry analysis of paraffin-embedded mouse heart using PLOD3 Rabbit polyclonal antibody (STJ112565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PLOD3 antibody (STJ112565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PLOD3 antibody (25-280) (STJ112565)

SKU:
STJ112565

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLOD3 (25-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLOD3
Gene ID: 8985
Uniprot ID: PLOD3_HUMAN
Immunogen Region: 25-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human PLOD3 (NP_001075.1).
Immunogen Sequence: SDRPRGRDPVNPEKLLVITV ATAETEGYLRFLRSAEFFNY TVRTLGLGEEWRGGDVARTV GGGQKVRWLKKEMEKYADRE DMIIMFVDSYDVILAGSPTE LLKKFVQSGSRLLFSAESFC WPEWGLAEQYPEVGTGKRFL NSGGFIGFATTIHQIVRQWK YKDDDDDQLFYTRLYLDPGL REKLSLNLDHKSRIFQNLNG ALDEVVLKFDRNRVRIRNVA YDTLPIVVHGNGPTKLQLN
Tissue Specificity Ubiquitous. Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle.
Function Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine. Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens (Probable). Essential for normal formation of basement membranes.
Protein Name Multifunctional Procollagen Lysine Hydroxylase And Glycosyltransferase Lh3 Includes - Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 3
Lysyl Hydroxylase 3
Lh3 - Procollagen Glycosyltransferase
Galactosylhydroxylysine-Glucosyltransferase
Procollagen Galactosyltransferase
Procollagen Glucosyltransferase
Database Links Reactome: R-HSA-1650814
Cellular Localisation Rough Endoplasmic Reticulum
Endoplasmic Reticulum Lumen
Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Lumenal Side
Secreted
Extracellular Space
The Majority Of The Secreted Protein Is Associated With The Extracellular Matrix
Alternative Antibody Names Anti-Multifunctional Procollagen Lysine Hydroxylase And Glycosyltransferase Lh3 Includes - Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 3 antibody
Anti-Lysyl Hydroxylase 3 antibody
Anti-Lh3 - Procollagen Glycosyltransferase antibody
Anti-Galactosylhydroxylysine-Glucosyltransferase antibody
Anti-Procollagen Galactosyltransferase antibody
Anti-Procollagen Glucosyltransferase antibody
Anti-PLOD3 antibody

Information sourced from Uniprot.org

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