• Western blot analysis of extracts of various cell lines, using PLOD1 antibody (STJ110228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using PLOD1 antibody (STJ110228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PLOD1 antibody (19-295) (STJ110228)

SKU:
STJ110228

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLOD1
Gene ID: 5351
Uniprot ID: PLOD1_HUMAN
Immunogen Region: 19-295
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 19-295 of human PLOD1 (NP_000293.2).
Immunogen Sequence: KGDAKPEDNLLVLTVATKET EGFRRFKRSAQFFNYKIQAL GLGEDWNVEKGTSAGGGQKV RLLKKALEKHADKEDLVILF ADSYDVLFASGPRELLKKFR QARSQVVFSAEELIYPDRRL ETKYPVVSDGKRFLGSGGFI GYAPNLSKLVAEWEGQDSDS DQLFYTKIFLDPEKREQINI TLDHRCRIFQNLDGALDEVV LKFEMGHVRARNLAYDTLPV LIHGNGPTKLQLNYLGNYI
Function Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Protein Name Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 1
Lysyl Hydroxylase 1
Lh1
Database Links Reactome: R-HSA-1650814
Cellular Localisation Rough Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Lumenal Side
Alternative Antibody Names Anti-Procollagen-Lysine -2-Oxoglutarate 5-Dioxygenase 1 antibody
Anti-Lysyl Hydroxylase 1 antibody
Anti-Lh1 antibody
Anti-PLOD1 antibody
Anti-LLH antibody
Anti-PLOD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance