STJ70664 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Plexin D1 antibody (Internal) (STJ70664)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70664-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Plexin D1 (Internal) is suitable for use in ELISA, Flow Cytometry and Immunofluorescence research applications.
ApplicationsPep-ELISA/FC/IF
HostGoat
ReactivityHuman/Dog/Pig/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
IHC:.
IF: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and U251cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of K562 ce

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolPLXND1
Gene ID23129
Uniprot IDPLXD1_HUMAN
Accession NumberNP_055918.2
Immunogen RegionInternal
Immunogen SequenceLAEPKKSHRQSH

Additional Info

Function Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature. Mediates anti-angiogenic signaling in response to SEMA3E.
Protein Name Plexin-D1
Database Links Reactome: R-HSA-416700
Reactome: R-HSA-9013508
Reactome: R-HSA-9696270
Cellular Localisation Cell Membrane
Single-Pass Membrane Protein
Cell Projection
Lamellipodium Membrane
Alternative Antibody Names Anti-Plexin-D1 antibody
Anti-PLXND1 antibody
Anti-KIAA0620 antibody

Information sourced from Uniprot.org

Citations

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