| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLD3 |
| Gene ID: |
23646 |
| Uniprot ID: |
PLD3_HUMAN |
| Immunogen Region: |
279-490 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 279-490 of human PLD3 (NP_001026866). |
| Immunogen Sequence: |
MEICLNGTPALAYLASAPPP LCPSGRTPDLKALLNVVDNA RSFIYVAVMNYLPTLEFSHP HRFWPAIDDGLRRATYERGV KVRLLISCWGHSEPSMRAFL LSLAALRDNHTHSDIQVKLF VVPADEAQARIPYARVNHNK YMVTERATYIGTSNWSGNYF TETAGTSLLVTQNGRGGLRS QLEAIFLRDWDSPYSHDLDT SADSVGNACRLL |
| Tissue Specificity | Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum. |
| Post Translational Modifications | N-glycosylated. Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex. Ubiquitinated at N-terminus. Ubiquitination mediates sorting into lysosomes. |
| Function | 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4. May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting. |
| Protein Name | 5'-3' Exonuclease Pld3Choline Phosphatase 3Hindiii K4l HomologHu-K4Phosphatidylcholine-Hydrolyzing Phospholipase D3Phospholipase D3Pld 3 |
| Database Links | Reactome: R-HSA-1483148Reactome: R-HSA-2029485 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinLysosome LumenEarly Endosome MembraneLate Endosome MembraneGolgi Apparatus MembraneEndosome MembraneLocalizes To Er-Associated Vesicles In Differentiating MyotubesThe Soluble Form In Lysosome Arises By Proteolytic Processing Of The Membrane-Bound FormColocalizes With App In Endosomes |
| Alternative Antibody Names | Anti-5'-3' Exonuclease Pld3 antibodyAnti-Choline Phosphatase 3 antibodyAnti-Hindiii K4l Homolog antibodyAnti-Hu-K4 antibodyAnti-Phosphatidylcholine-Hydrolyzing Phospholipase D3 antibodyAnti-Phospholipase D3 antibodyAnti-Pld 3 antibodyAnti-PLD3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
