• Western blot analysis of extracts of Rat lung, using PLCB2 antibody (STJ110440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of HEL cells, using PLCB2 antibody (STJ110440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of HEL cells, using PLCB2 antibody (STJ110440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Rat lung, using PLCB2 antibody (STJ110440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PLCB2 antibody (1-199) (STJ110440)

SKU:
STJ110440

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCB2
Gene ID: 5330
Uniprot ID: PLCB2_HUMAN
Immunogen Region: 1-199
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human PLCB2 (NP_004564.2).
Immunogen Sequence: MSLLNPVLLPPKVKAYLSQG ERFIKWDDETTVASPVILRV DPKGYYLYWTYQSKEMEFLD ITSIRDTRFGKFAKMPKSQK LRDVFNMDFPDNSFLLKTLT VVSGPDMVDLTFHNFVSYKE NVGKAWAEDVLALVKHPLTA NASRSTFLDKILVKLKMQLN SEGKIPVKNFFQMFPADRKR VEAALSACHLPKGKNDAIN
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-2
Phosphoinositide Phospholipase C-Beta-2
Phospholipase C-Beta-2
Plc-Beta-2
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-1855204
Reactome: R-HSA-399997
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-418217
Reactome: R-HSA-434316
Reactome: R-HSA-500657
Reactome: R-HSA-9717207
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-2 antibody
Anti-Phosphoinositide Phospholipase C-Beta-2 antibody
Anti-Phospholipase C-Beta-2 antibody
Anti-Plc-Beta-2 antibody
Anti-PLCB2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance