| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase (49-98) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PKMYT1 |
| Gene ID: | 9088 |
| Uniprot ID: | PMYT1_HUMAN |
| Immunogen Region: | 49-98 |
| Specificity: | Myt 1 Polyclonal Antibody detects endogenous levels of Myt 1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PKMYT1. AA range:49-98 |
| Post Translational Modifications | Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity. |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. |
| Protein Name | Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory KinaseMyt1 Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69273Reactome: R-HSA-69478 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinGolgi Apparatus Membrane |
| Alternative Antibody Names | Anti-Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory Kinase antibodyAnti-Myt1 Kinase antibodyAnti-PKMYT1 antibodyAnti-MYT1 antibody |
Information sourced from Uniprot.org