| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | PKD1 |
| Gene ID: | 5310 |
| Uniprot ID: | PKD1_HUMAN |
| Immunogen Region: | 3700-3860 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 3700-3860 of human PKD1 (NP_001009944.2). |
| Immunogen Sequence: | RLQSAIKQELHSRAFLAITR SEELWPWMAHVLLPYVHGNQ SSPELGPPRLRQVRLQEALY PDPPGPRVHTCSAAGGFSTS DYDVGWESPHNGSGTWAYSA PDLLGAWSWGSCAVYDSGGY VQELGLSLEESRDRLRFLQL HNWLDNRSRAVFLELTRYSP A |
| Post Translational Modifications | N-glycosylated. After synthesis, undergoes cleavage between Leu-3048 and Thr-3049 in the GPS region of the GAIN-B domain. Cleavage at the GPS region occurs through a cis-autoproteolytic mechanism involving an ester-intermediate via N-O acyl rearrangement. This process takes place in the early secretory pathway, depends on initial N-glycosylation, and requires the REJ domain. There is evidence that cleavage at GPS region is incomplete. Uncleaved and cleaved products may have different functions in vivo. |
| Function | Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Both PKD1 and PKD2 are required for channel activity. Involved in renal tubulogenesis. Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Acts as a regulator of cilium length, together with PKD2. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. May be an ion-channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Likely to be involved with polycystin-1-interacting protein 1 in the detection, sequestration and exocytosis of senescent mitochondria. |
| Protein Name | Polycystin-1 Pc1 Autosomal Dominant Polycystic Kidney Disease 1 Protein |
| Database Links | Reactome: R-HSA-5620916 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cell Projection Cilium Endoplasmic Reticulum Golgi Apparatus Vesicle Secreted Extracellular Exosome Pkd1 Localization To The Plasma And Ciliary Membranes Requires Pkd2 Is Independent Of Pkd2 Channel Activity And Involves Stimulation Of Pkd1 Autoproteolytic Cleavage At The Gps Region Of The Gain-B Domain Pkd1:Pkd2 Interaction Is Required To Reach The Golgi Apparatus From Endoplasmic Reticulum And Then Traffic To The Cilia Ciliary Localization Of Pkd1 Requires Bbs1 And Arl6/Bbs3 Cell Surface Localization Requires Ganab Detected On Migrasomes And On Extracellular Exosomes In Urine |
| Alternative Antibody Names | Anti-Polycystin-1 antibody Anti-Pc1 antibody Anti-Autosomal Dominant Polycystic Kidney Disease 1 Protein antibody Anti-PKD1 antibody |
Information sourced from Uniprot.org