Anti-PKC Alpha antibody (Central Region) [M237] (STJA0041317)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenClone (M237) was generated from a recombinant human PKC Alpha that included amino acids residues in the central region. This region is highly conserved in rat and mouse PKC Alpha, and has homology to conserved regions in PKC Beta.
General Information
| Short Description | Mouse monoclonal anti-PKC Alpha (Central Region) for use in ELISA, WB, ICC, IHC and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IHC/IP |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M237 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Protein A Purified |
| Dilution Range | WB 1:1000IHC 1:500ICC 1:300IP 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20ยฐC is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20ยฐC. |
Target Information
| Gene Symbol | PRKCA |
| Gene ID | 5578 |
| Uniprot ID | KPCA_HUMAN |
| Immunogen | Clone (M237) was generated from a recombinant human PKC Alpha that included amino acids residues in the central region. This region is highly conserved in rat and mouse PKC Alpha, and has homology to conserved regions in PKC Beta. |
| Immunogen Region | Central Region |
| Immunogen Sequence | Human |
| Specificity | This antibody detects an 82kDa* protein corresponding to the molecular mass of PKC Alpha on SDS-PAGE immunoblots of neonatal rat brain and adult mouse brain lysates. |
Additional Info
| Post Translational Modifications | In response to growth factors, phosphorylated at Thr-631 and Ser-657 by the mTORC2 complex, promoting autophosphorylation and activation of PRKCA. |
| Function | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteasomal degradation. Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4. Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking. |
| Protein Name | Protein Kinase C Alpha TypePkc-APkc-Alpha |
| Database Links | Reactome: R-HSA-111933Reactome: R-HSA-114516Reactome: R-HSA-1250196Reactome: R-HSA-1433557Reactome: R-HSA-1433559Reactome: R-HSA-2179392Reactome: R-HSA-2514859Reactome: R-HSA-3000170Reactome: R-HSA-399997Reactome: R-HSA-4086398Reactome: R-HSA-416993Reactome: R-HSA-418597Reactome: R-HSA-4419969Reactome: R-HSA-450520Reactome: R-HSA-5099900Reactome: R-HSA-5218921Reactome: R-HSA-5668599Reactome: R-HSA-76005Reactome: R-HSA-8853659Reactome: R-HSA-9010642 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinMitochondrion MembraneNucleus |
| Alternative Antibody Names | Anti-Protein Kinase C Alpha Type antibodyAnti-Pkc-A antibodyAnti-Pkc-Alpha antibodyAnti-PRKCA antibodyAnti-PKCA antibodyAnti-PRKACA antibody |
Information sourced from Uniprot.org