| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat/Pig |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-cAMP-dependent protein kinase catalytic subunit alpha and cAMP-dependent protein kinase catalytic subunit beta and cAMP-dependent protein kinase catalytic subunit gamma (166-215 aa) is suitable for use in Western Blot, |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRKACBPRKACG556755685566KAPCB_HUMANKAPCG_HUMANKAPCA_HUMAN |
| Immunogen Region: |
166-215 aa |
| Specificity: |
PKA Alpha/Beta/Gamma cat Polyclonal Antibody detects endogenous levels of PKA Alpha/Beta/Gamma cat protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human PKA alpha/beta CAT at the amino acid range 166-215 |
| Post Translational Modifications | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR.this increases catalytic efficiency. |
| Function | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in chondrogenesis by mediating phosphorylation of SOX9. Involved in the regulation of platelets in response to thrombin and collagen.maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Phosphorylates HSF1.this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock. Isoform 2: Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. |
| Protein Name | Camp-Dependent Protein Kinase Catalytic Subunit AlphaPka C-Alpha |
| Database Links | Reactome: R-HSA-111931Reactome: R-HSA-163358Reactome: R-HSA-163560Reactome: R-HSA-163615Reactome: R-HSA-164378Reactome: R-HSA-180024Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-381676Reactome: R-HSA-392517Reactome: R-HSA-422356Reactome: R-HSA-432040Reactome: R-HSA-4420097Reactome: R-HSA-442720Reactome: R-HSA-512988Reactome: R-HSA-5578775Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5610787Reactome: R-HSA-5620912Reactome: R-HSA-5621575Reactome: R-HSA-5687128Reactome: R-HSA-8853659Reactome: R-HSA-8854518Reactome: R-HSA-8963896Reactome: R-HSA-9010642Reactome: R-HSA-9022535Reactome: R-HSA-9022692Reactome: R-HSA-9634600Reactome: R-HSA-9660821Reactome: R-HSA-9664323Reactome: R-HSA-983231 |
| Cellular Localisation | CytoplasmCell MembraneNucleusMitochondrionMembraneLipid-AnchorTranslocates Into The Nucleus (Monomeric Catalytic Subunit)The Inactive Holoenzyme Is Found In The CytoplasmDistributed Throughout The Cytoplasm In Meiotically Incompetent OocytesAssociated To Mitochondrion As Meiotic Competence Is AcquiredAggregates Around The Germinal Vesicles (Gv) At The Immature Gv Stage OocytesColocalizes With Hsf1 In Nuclear Stress Bodies (Nsbs) Upon Heat ShockIsoform 2: Cell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeExpressed In The Midpiece Region Of The Sperm FlagellumColocalizes With Mroh2b And Tcp11 On The Acrosome And Tail Regions In Round Spermatids And Spermatozoa Regardless Of The Capacitation Status Of The Sperm |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Catalytic Subunit Alpha antibodyAnti-Pka C-Alpha antibodyAnti-PRKACA antibodyAnti-PKACA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
