| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIP4K2A (120-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIP4K2A |
| Gene ID: |
5305 |
| Uniprot ID: |
PI42A_HUMAN |
| Immunogen Region: |
120-350 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2). |
| Immunogen Sequence: |
APLPNDSQARSGARFHTSYD KRYIIKTITSEDVAEMHNIL KKYHQYIVECHGITLLPQFL GMYRLNVDGVEIYVIVTRNV FSHRLSVYRKYDLKGSTVAR EASDKEKAKELPTLKDNDFI NEGQKIYIDDNNKKVFLEKL KKDVEFLAQLKLMDYSLLVG IHDVERAEQEEVECEENDGE EEGESDGTHPVGTPPDSPGN TLNSSPPLAPGEFDPNIDVY GIKCHENSPRK |
| Tissue Specificity | Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine. |
| Post Translational Modifications | Phosphorylated in tyrosines. Phosphorylation is induced by light and increases kinase activity. |
| Function | Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level. In collaboration with PIP4K2B, has a role in mediating autophagy in times of nutrient stress. Required for autophagosome-lysosome fusion and the regulation of cellular lipid metabolism. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. Negatively regulates insulin signaling through a catalytic-independent mechanism. PIP4Ks interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3. |
| Protein Name | Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-AlphaDiphosphoinositide Kinase 2-AlphaPip5kiiiPhosphatidylinositol 5-Phosphate 4-KinasePi5p4kalphaPhosphatidylinositol 5-Phosphate 4-Kinase Type Ii AlphaPi(5p 4-Kinase Type Ii AlphaPip4kii-AlphaPtdins(4p-5-Kinase B IsoformPtdins(4p-5-Kinase C IsoformPtdins(5p-4-Kinase Isoform 2-Alpha |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-6811555Reactome: R-HSA-6811558Reactome: R-HSA-8847453 |
| Cellular Localisation | Cell MembraneNucleusLysosomeCytoplasmPhotoreceptor Inner SegmentCell ProjectionCiliumPhotoreceptor Outer SegmentMay Translocate From The Cytosol To The Cell Membrane Upon Activation Of Tyrosine PhosphorylationMay Translocate From The Inner To The Outer Segments Of The Rod Photoreceptor Cells In Response To LightLocalization To The Nucleus Is Modulated By The Interaction With Pip4k2b |
| Alternative Antibody Names | Anti-Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Alpha antibodyAnti-1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Alpha antibodyAnti-Diphosphoinositide Kinase 2-Alpha antibodyAnti-Pip5kiii antibodyAnti-Phosphatidylinositol 5-Phosphate 4-Kinase antibodyAnti-Pi5p4kalpha antibodyAnti-Phosphatidylinositol 5-Phosphate 4-Kinase Type Ii Alpha antibodyAnti-Pi(5p 4-Kinase Type Ii Alpha antibodyAnti-Pip4kii-Alpha antibodyAnti-Ptdins(4p-5-Kinase B Isoform antibodyAnti-Ptdins(4p-5-Kinase C Isoform antibodyAnti-Ptdins(5p-4-Kinase Isoform 2-Alpha antibodyAnti-PIP4K2A antibodyAnti-PI5P4KA antibodyAnti-PIP5K2 antibodyAnti-PIP5K2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
