Anti-PIN1 antibody (1-163) (STJ115621)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PIN1 (1-163) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIN1 |
| Gene ID | 5300 |
| Uniprot ID | PIN1_HUMAN |
| Immunogen Region | 1-163 |
| Immunogen Sequence | MADEEKLPPGWEKRMSRSSG RVYYFNHITNASQWERPSGN SSSGGKNGQGEPARVRCSHL LVKHSQSRRPSSWRQEKITR TKEEALELINGYIQKIKSGE EDFESLASQFSDCSSAKARG DLGAFSRGQMQKPFEDASFA LRTGEMSGPVFTDSGIHIIL RTE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human Pin1 (NP_006212.1). |
Additional Info
| Tissue Specificity | Expressed in immune cells in the lung (at protein level). The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells. |
| Post Translational Modifications | Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation. Ser-71 is dephosphorylated upon IL33-stimulation of dendritic cells. |
| Function | Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells. Catalyzes cis-trans isomerization of phosphorylated phosphoglycerate kinase PGK1 under hypoxic conditions to promote its binding to the TOM complex and targeting to the mitochondrion. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 1Peptidyl-Prolyl Cis-Trans Isomerase Pin1Ppiase Pin1Rotamase Pin1 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-5668599Reactome: R-HSA-6804756Reactome: R-HSA-6811555Reactome: R-HSA-936440 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmColocalizes With Nek6 In The NucleusMainly Localized In The Nucleus But Phosphorylation At Ser-71 By Dapk1 Results In Inhibition Of Its Nuclear Localization |
| Alternative Antibody Names | Anti-Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 1 antibodyAnti-Peptidyl-Prolyl Cis-Trans Isomerase Pin1 antibodyAnti-Ppiase Pin1 antibodyAnti-Rotamase Pin1 antibodyAnti-PIN1 antibody |
Information sourced from Uniprot.org