| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIK3CA |
| Gene ID: | 5290 |
| Uniprot ID: | PK3CA_HUMAN |
| Immunogen Region: | 300-600 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PIK3CA (NP_006209.2). |
| Immunogen Sequence: | DCFTMPSYSRRISTATPYMN GETSTKSLWVINSALRIKIL CATYVNVNIRDIDKIYVRTG IYHGGEPLCDNVNTQRVPCS NPRWNEWLNYDIYIPDLPRA ARLCLSICSVKGRKGAKEEH CPLAWGNINLFDYTDTLVSG KMALNLWPVPHGLEDLLNPI GVTGSNPNKETPCLELEFDW FSSVVKFPDMSVIEEHANWS VSREAGFSYSHAGLSNRLAR DNELRENDKEQLKAISTRD |
| Function | Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others. Plays a role in the positive regulation of phagocytosis and pinocytosis. |
| Protein Name | Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform Pi3-Kinase Subunit Alpha Pi3k-Alpha Pi3kalpha Ptdins-3-Kinase Subunit Alpha Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Alpha Ptdins-3-Kinase Subunit P110-Alpha P110alpha Phosphoinositide 3-Kinase Alpha Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide Serine/Threonine Protein Kinase Pik3ca |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-114604 Reactome: R-HSA-1236382 Reactome: R-HSA-1250342 Reactome: R-HSA-1257604 Reactome: R-HSA-1433557 Reactome: R-HSA-1660499 Reactome: R-HSA-180292 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-1963642 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-202424 Reactome: R-HSA-2029485 Reactome: R-HSA-210993 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-2730905 Reactome: R-HSA-373753 Reactome: R-HSA-388841 Reactome: R-HSA-389357 Reactome: R-HSA-416476 Reactome: R-HSA-4420097 Reactome: R-HSA-512988 Reactome: R-HSA-5637810 Reactome: R-HSA-5654689 Reactome: R-HSA-5654695 Reactome: R-HSA-5654710 Reactome: R-HSA-5654720 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851907 Reactome: R-HSA-8853659 Reactome: R-HSA-9009391 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9027276 Reactome: R-HSA-9028335 Reactome: R-HSA-912526 Reactome: R-HSA-912631 Reactome: R-HSA-9603381 Reactome: R-HSA-9607240 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9680350 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9725370 Reactome: R-HSA-9842640 Reactome: R-HSA-9842663 |
| Alternative Antibody Names | Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform antibody Anti-Pi3-Kinase Subunit Alpha antibody Anti-Pi3k-Alpha antibody Anti-Pi3kalpha antibody Anti-Ptdins-3-Kinase Subunit Alpha antibody Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Alpha antibody Anti-Ptdins-3-Kinase Subunit P110-Alpha antibody Anti-P110alpha antibody Anti-Phosphoinositide 3-Kinase Alpha antibody Anti-Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide antibody Anti-Serine/Threonine Protein Kinase Pik3ca antibody Anti-PIK3CA antibody |
Information sourced from Uniprot.org