• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g PIK3C2A antibody (STJ11104405). Western blot was performed from the immunoprecipitate using PIK3C2A antibody (STJ11104405) at a dilution of 1:1000.
  • Immunofluorescence analysis of C6 cells using PIK3C2A Rabbit polyclonal antibody (STJ11104405) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PIK3C2A in paraffin-embedded human liver damage using PIK3C2A Rabbit polyclonal antibody (STJ11104405) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PIK3C2A in paraffin-embedded rat heart using PIK3C2A Rabbit polyclonal antibody (STJ11104405) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PIK3C2A in paraffin-embedded mouse heart using PIK3C2A Rabbit polyclonal antibody (STJ11104405) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PIK3C2A Rabbit polyclonal antibody (STJ11104405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIK3C2A antibody (1-200) (STJ11104405)

SKU:
STJ11104405

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha (1-200) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3C2A
Gene ID: 5286
Uniprot ID: P3C2A_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).
Immunogen Sequence: MAQISSNSGFKECPSSHPEP TRAKDVDKEEALQMEAEALA KLQKDRQVTDNQRGFELSSS TRKKAQVYNKQDYDLMVFPE SDSQKRALDIDVEKLTQAEL EKLLLDDSFETKKTPVLPVT PILSPSFSAQLYFRPTIQRG QWPPGLPGPSTYALPSIYPS TYSKQAAFQNGFNPRMPTFP STEPIYLSLPGQSPYFSYPL
Tissue Specificity Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals.
Post Translational Modifications Phosphorylated upon insulin stimulation.which may lead to enzyme activation. Phosphorylated on Ser-259 during mitosis and upon UV irradiation.which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell.
Function Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components.
Protein Name Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha
Pi3k-C2-Alpha
Ptdins-3-Kinase C2 Subunit Alpha
Phosphoinositide 3-Kinase-C2-Alpha
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660514
Reactome: R-HSA-1660516
Reactome: R-HSA-1660517
Reactome: R-HSA-432722
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Nucleus
Cytoplasm
Golgi Apparatus
Trans-Golgi Network
Inserts Preferentially Into Membranes Containing Ptdins(4
5)P2
Associated With Rna-Containing Structures
Alternative Antibody Names Anti-Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha antibody
Anti-Pi3k-C2-Alpha antibody
Anti-Ptdins-3-Kinase C2 Subunit Alpha antibody
Anti-Phosphoinositide 3-Kinase-C2-Alpha antibody
Anti-PIK3C2A antibody

Information sourced from Uniprot.org

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