Western blot analysis of lysates from rat kidney, using PIGX Rabbit polyclonal antibody (STJ117655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIGX antibody (80-220) (STJ117655)

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STJ117655
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGX
Gene ID: 54965
Uniprot ID: PIGX_HUMAN
Immunogen Region: 80-220
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 80-220 of human PIGX (NP_060331.3).
Immunogen Sequence: LIKQDIPAGLYVDPYELASL RERNITEAVMVSENFDIEAP NYLSKESEVLIYARRDSQCI DCFQAFLPVHCRYHRPHSED GEASIVVNNPDLLMFCDQEF PILKCWAHSEVAAPCALENE DICQWNKMKYKSVYKNVILQ V
Function Stabilizing subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1->4)-alpha-D-GlcN-(1->6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
Protein Name Gpi Alpha-1 -4-Mannosyltransferase I - Stabilizing Subunit
Phosphatidylinositol-Glycan Biosynthesis Class X Protein
Pig-X
Database Links Reactome: R-HSA-162710
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Gpi Alpha-1 -4-Mannosyltransferase I - Stabilizing Subunit antibody
Anti-Phosphatidylinositol-Glycan Biosynthesis Class X Protein antibody
Anti-Pig-X antibody
Anti-PIGX antibody

Information sourced from Uniprot.org

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