• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from 293, COLO, HUVEC, and Jurkat cells, using PIGX Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PIG-X Polyclonal Antibody diluted at 1：1000

Anti-PIGX antibody (183-232 aa) (STJ95091)

SKU:
STJ95091

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phosphatidylinositol-glycan biosynthesis class X protein (183-232 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGX
Gene ID: 54965
Uniprot ID: PIGX_HUMAN
Immunogen Region: 183-232 aa
Specificity: PIG-X Polyclonal Antibody detects endogenous levels of PIG-X protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PIGX at the amino acid range 183-232
Function Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
Protein Name Phosphatidylinositol-Glycan Biosynthesis Class X Protein
Pig-X
Database Links Reactome: R-HSA-162710
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Phosphatidylinositol-Glycan Biosynthesis Class X Protein antibody
Anti-Pig-X antibody
Anti-PIGX antibody

Information sourced from Uniprot.org

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