| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 SUMO-protein ligase PIAS3 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIAS3 |
| Gene ID: | 10401 |
| Uniprot ID: | PIAS3_HUMAN |
| Immunogen Region: | 10-59 aa |
| Specificity: | PIAS 3 Polyclonal Antibody detects endogenous levels of PIAS 3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PIAS3 at the amino acid range 10-59 |
| Post Translational Modifications | Sumoylated. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus. |
| Protein Name | E3 Sumo-Protein Ligase Pias3E3 Sumo-Protein Transferase Pias3Protein Inhibitor Of Activated Stat Protein 3 |
| Database Links | Reactome: R-HSA-3232118Reactome: R-HSA-3899300Reactome: R-HSA-4090294Reactome: R-HSA-4615885Reactome: R-HSA-4755510Reactome: R-HSA-5696395 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleColocalizes With Mitf In The NucleusColocalizes With Gfi1 In Nuclear DotsColocalizes With Sumo1 In Nuclear Granules |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias3 antibodyAnti-E3 Sumo-Protein Transferase Pias3 antibodyAnti-Protein Inhibitor Of Activated Stat Protein 3 antibodyAnti-PIAS3 antibody |
Information sourced from Uniprot.org