• Western blot analysis of lysates from 1) 293, 2) Rat kidney, 3) HELA cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of lysates from 293 cells, treated with UV 5', using PIAS3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using PIAS 3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PIAS 3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PIAS3 antibody (10-59 aa) (STJ95083)

SKU:
STJ95083

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 SUMO-protein ligase PIAS3 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIAS3
Gene ID: 10401
Uniprot ID: PIAS3_HUMAN
Immunogen Region: 10-59 aa
Specificity: PIAS 3 Polyclonal Antibody detects endogenous levels of PIAS 3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PIAS3 at the amino acid range 10-59
Post Translational Modifications Sumoylated.
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.
Protein Name E3 Sumo-Protein Ligase Pias3
E3 Sumo-Protein Transferase Pias3
Protein Inhibitor Of Activated Stat Protein 3
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-3899300
Reactome: R-HSA-4090294
Reactome: R-HSA-4615885
Reactome: R-HSA-4755510
Reactome: R-HSA-5696395
Cellular Localisation Cytoplasm
Nucleus
Nucleus Speckle
Colocalizes With Mitf In The Nucleus
Colocalizes With Gfi1 In Nuclear Dots
Colocalizes With Sumo1 In Nuclear Granules
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Pias3 antibody
Anti-E3 Sumo-Protein Transferase Pias3 antibody
Anti-Protein Inhibitor Of Activated Stat Protein 3 antibody
Anti-PIAS3 antibody

Information sourced from Uniprot.org

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