• Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human pancreas, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293Tmouse brainusing p-14-3-3 Theta/Tau (S232) antibody. Antibody was diluted at 1:500
  • Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-YWHAQ-Ser232 antibody (196-245 aa) (STJ91063)

SKU:
STJ91063

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-14-3-3 protein theta-Ser232 (196-245 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAQ
Gene ID: 10971
Uniprot ID: 1433T_HUMAN
Immunogen Region: 196-245 aa
Specificity: Phospho-14-3-3 Theta/Tau (S232) Polyclonal Antibody detects endogenous levels of 14-3-3 Theta/Tau protein only when phosphorylated at S232.
Immunogen: The antiserum was produced against synthesized peptide derived from the human 14-3-3 thet/tau around the phosphorylation site of Ser232 at the amino acid range 196-245
Post Translational Modifications Ser-232 is probably phosphorylated by CK1.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Protein Name 14-3-3 Protein Theta
14-3-3 Protein T-Cell
14-3-3 Protein Tau
Protein Hs1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
In Neurons
Axonally Transported To The Nerve Terminals
Alternative Antibody Names Anti-14-3-3 Protein Theta antibody
Anti-14-3-3 Protein T-Cell antibody
Anti-14-3-3 Protein Tau antibody
Anti-Protein Hs1 antibody
Anti-YWHAQ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance