Anti-Phospho-USP28-Ser714 antibody (STJA0009969)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human USP28 (Phospho-Ser714)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Ubiquitin carboxyl-terminal hydrolase 28-Ser714 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP28 |
| Gene ID | 57646 |
| Uniprot ID | UBP28_HUMAN |
| Immunogen | Synthesized peptide derived from human USP28 (Phospho-Ser714) |
| Specificity | This antibody detects endogenous levels of USP28 (Phospho-Ser714) at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | Degraded upon nickel ion level or hypoxia exposure. Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1. |
| Function | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 28Deubiquitinating Enzyme 28Ubiquitin Thioesterase 28Ubiquitin-Specific-Processing Protease 28 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusNucleoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 28 antibodyAnti-Deubiquitinating Enzyme 28 antibodyAnti-Ubiquitin Thioesterase 28 antibodyAnti-Ubiquitin-Specific-Processing Protease 28 antibodyAnti-USP28 antibodyAnti-KIAA1515 antibody |
Information sourced from Uniprot.org