Host: | Rabbit |
Applications: | WB/ELISA |
Reactivity: | Human/Rat/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-Phospho-DNA topoisomerase 2-alpha-Thr1343 (1311-1360 aa) is suitable for use in Western Blot and ELISA research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000ELISA 1:5000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | TOP2A |
Gene ID: | 7153 |
Uniprot ID: | TOP2A_HUMAN |
Immunogen Region: | 1311-1360 aa |
Specificity: | Phospho-Topo II Alpha (T1343) Polyclonal Antibody detects endogenous levels of Topo II Alpha protein only when phosphorylated at T1343. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human TOP2A around the phosphorylation site of Thr1343 at the amino acid range 1311-1360 |
Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
Protein Name | Dna Topoisomerase 2-AlphaDna Topoisomerase Ii - Alpha Isozyme |
Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-4615885 |
Cellular Localisation | CytoplasmNucleusNucleoplasmNucleolus |
Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibodyAnti-Dna Topoisomerase Ii - Alpha Isozyme antibodyAnti-TOP2A antibodyAnti-TOP2 antibody |
Information sourced from Uniprot.org