• Immunohistochemistry analysis of paraffin-embedded human brain, using TOP2A (Phospho-Ser1525) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 5', using TOP2A (Phospho-Ser1525) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293T 3T3 COLO using p-Topo II Alpha (S1525) antibody. Antibody was diluted at 1:1000

Anti-Phospho-TOP2A-Ser1525 antibody (1482-1531 aa) (STJ90996)

SKU:
STJ90996

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DNA topoisomerase 2-alpha-Ser1525 (1482-1531 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TOP2A
Gene ID: 7153
Uniprot ID: TOP2A_HUMAN
Immunogen Region: 1482-1531 aa
Specificity: Phospho-Topo II Alpha (S1525) Polyclonal Antibody detects endogenous levels of Topo II Alpha protein only when phosphorylated at S1525.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TOP2A around the phosphorylation site of Ser1525 at the amino acid range 1482-1531
Post Translational Modifications Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated.
Function Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation.
Protein Name Dna Topoisomerase 2-Alpha
Dna Topoisomerase Ii - Alpha Isozyme
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-4615885
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Alternative Antibody Names Anti-Dna Topoisomerase 2-Alpha antibody
Anti-Dna Topoisomerase Ii - Alpha Isozyme antibody
Anti-TOP2A antibody
Anti-TOP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance