Anti-Phospho-TK1-Ser13 antibody (1-50 aa) (STJ90834)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TK around the phosphorylation site of Ser13 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Thymidine kinase, cytosolic-Ser13 (1-50 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Pig samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TK1 |
| Gene ID | 7083 |
| Uniprot ID | KITH_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TK around the phosphorylation site of Ser13 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | Phospho-Thymidine Kinase (S13) Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein only when phosphorylated at S13. |
Additional Info
| Function | Cell-cycle-regulated enzyme of importance in nucleotide metabolism. Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate. Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT). |
| Protein Name | Thymidine Kinase - Cytosolic |
| Database Links | Reactome: R-HSA-69205Reactome: R-HSA-73614 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Thymidine Kinase - Cytosolic antibodyAnti-TK1 antibody |
Information sourced from Uniprot.org