• Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Pan Phospho-Threonine Rabbit monoclonal antibody (STJ11105878) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of lysates from A-431 or A 431 treat with Calyculin A, using Pan Phospho-Threonine Rabbit monoclonal antibody (STJ11105878) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-Threonine antibody [S5878RM] (STJ11105878)

SKU:
STJ11105878

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Threonine is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5878RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around of human Threonine.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance