| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Tyrosine 3-monooxygenase-Ser19 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TH |
| Gene ID: |
7054 |
| Uniprot ID: |
TY3H_HUMAN |
| Immunogen Region: |
10-59 aa |
| Specificity: |
Phospho-TH (S19) Polyclonal Antibody detects endogenous levels of TH protein only when phosphorylated at S19. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Tyrosine Hydroxylase around the phosphorylation site of Ser19 at the amino acid range 10-59 |
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity. |
| Protein Name | Tyrosine 3-MonooxygenaseTyrosine 3-HydroxylaseTh |
| Database Links | Reactome: R-HSA-209905 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusCell ProjectionAxonCytoplasmic VesicleSecretory VesicleSynaptic VesicleWhen Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic DistributionExpressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibodyAnti-Tyrosine 3-Hydroxylase antibodyAnti-Th antibodyAnti-TH antibodyAnti-TYH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
