• Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (STJ113483) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (STJ113483) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-SYK/ZAP70-Y319 antibody (STJ113483)

SKU:
STJ113483

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZAP70
SYK
Gene ID: 7535
6850
Uniprot ID: ZAP70_HUMAN
KSYK_HUMAN
Specificity: A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2).
Immunogen Sequence: SPYAD
Tissue Specificity P43403 - Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
P43405 - Widely expressed in hematopoietic cells (at protein level). Expressed in neutrophils (at protein level). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Zap-70 antibody
Anti-70 Kda Zeta-Chain Associated Protein antibody
Anti-Syk-Related Tyrosine Kinase antibody
Anti-ZAP70 antibody
Anti-SRK antibody
Anti-Tyrosine-Protein Kinase Syk antibody
Anti-Spleen Tyrosine Kinase antibody
Anti-P72-Syk antibody
Anti-SYK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance