• Western blot analysis of extracts of various cell lines, using Phospho-STK3/STK4-T180/T183 rabbit polyclonal antibody (STJ11101128) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-STK3/STK4-T180/T183 antibody (STJ11101128)

SKU:
STJ11101128

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK4
STK3
Gene ID: 6789
6788
Uniprot ID: STK4_HUMAN
STK3_HUMAN
Specificity: A synthetic phosphorylated peptide around T180 of human STK3/STK4STK3 (NP_006272.2).
Immunogen Sequence: RNTVI
Tissue Specificity Q13043 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
Q13188 - Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 4 antibody
Anti-Mammalian Ste20-Like Protein Kinase 1 antibody
Anti-Mst-1 antibody
Anti-Ste20-Like Kinase Mst1 antibody
Anti-Serine/Threonine-Protein Kinase Krs-2 Cleaved Into - Serine/Threonine-Protein Kinase 4 37kda Subunit antibody
Anti-Mst1/N - Serine/Threonine-Protein Kinase 4 18kda Subunit antibody
Anti-Mst1/C antibody
Anti-STK4 antibody
Anti-KRS2 antibody
Anti-MST1 antibody
Anti-Serine/Threonine-Protein Kinase 3 antibody
Anti-Mammalian Ste20-Like Protein Kinase 2 antibody
Anti-Mst-2 antibody
Anti-Ste20-Like Kinase Mst2 antibody
Anti-Serine/Threonine-Protein Kinase Krs-1 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda Subunit antibody
Anti-Mst2/N - Serine/Threonine-Protein Kinase 3 20kda Subunit antibody
Anti-Mst2/C antibody
Anti-STK3 antibody
Anti-KRS1 antibody
Anti-MST2 antibody

Information sourced from Uniprot.org

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