• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SRF-Thr159 antibody (126-175 aa) (STJ91269)

SKU:
STJ91269

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serum response factor-Thr159 (126-175 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRF
Gene ID: 6722
Uniprot ID: SRF_HUMAN
Immunogen Region: 126-175 aa
Specificity: Phospho-SRF (T159) Polyclonal Antibody detects endogenous levels of SRF protein only when phosphorylated at T159.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SRF around the phosphorylation site of Thr159 at the amino acid range 126-175
Function SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Protein Name Serum Response Factor
Srf
Database Links Reactome: R-HSA-5663220
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9733709
Reactome: R-HSA-9768777
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Serum Response Factor antibody
Anti-Srf antibody
Anti-SRF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance