• Western blot analysis of extracts of HeLa cells, using Phospho-SRF-S103 antibody (STJ117927) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of Phospho-SRF-S103 in paraffin-embedded mouse spleen using Phospho-SRF-S103 Rabbit polyclonal antibody (STJ117927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using Phospho-SRF-S103 antibody (STJ117927) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-SRF-S103 in paraffin-embedded human gastric cancer using Phospho-SRF-S103 Rabbit polyclonal antibody (STJ117927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-SRF-S103 antibody (STJ117927) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-SRF-S103 in paraffin-embedded rat spleen using Phospho-SRF-S103 Rabbit polyclonal antibody (STJ117927) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-SRF-S103 antibody (STJ117927) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from HeLa cells, using Phospho-SRF-S103 Rabbit polyclonal antibody (STJ117927) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-SRF-S103 antibody (STJ117927)

SKU:
STJ117927

$174.83 - $863.33
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SRF-S103 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRF
Gene ID: 6722
Uniprot ID: SRF_HUMAN
Immunogen: A synthetic phosphorylated peptide around S103 of human SRF (NP_003122.1).
Immunogen Sequence: SLSEM
Post Translational Modifications Phosphorylated by PRKDC.
Function SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Protein Name Serum Response Factor
Srf
Database Links Reactome: R-HSA-5663220
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9733709
Reactome: R-HSA-9768777
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Serum Response Factor antibody
Anti-Srf antibody
Anti-SRF antibody

Information sourced from Uniprot.org

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