| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Sequestosome-1-Ser403 is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SQSTM1 |
| Gene ID: |
8878 |
| Uniprot ID: |
SQSTM_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human Mouse Rat SQSTM1/p62 (phospho-Ser403) |
| Immunogen: |
Synthesized phosho peptide around human SQSTM1 (Ser403) |
| Post Translational Modifications | Phosphorylation at Ser-407 by ULK1 destabilizes the UBA dimer interface and increases binding affinity to ubiquitinated proteins. Phosphorylation at Ser-407 also primes for subsequent phosphorylation at Ser-403. Phosphorylation at Ser-403 by CK2 or ULK1 promotes binding to ubiquitinated proteins by increasing the affinity between the UBA domain and polyubiquitin chains. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by ULK1 promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. Phosphorylated in vitro by TTN. Ubiquitinated by UBE2J1 and RNF26 at Lys-435: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Ubiquitination by UBE2D2 and UBE2D3 increases its ability to bind polyubiquitin chains by destabilizing the UBA dimer interface. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication. Acetylated at Lys-420 and Lys-435 by KAT5/TIP60, promotes activity by destabilizing the UBA dimer interface and increases binding affinity to ubiquitinated proteins. Deacetylated by HDAC6. Palmitoylation at Cys-289 and Cys-290 by ZDHHC19 is required for efficient autophagic degradation of SQSTM1-cargo complexes by promoting affinity for ATG8 proteins and recruitment of p62 bodies to autophagosomes. Dealmitoylated at Cys-289 and Cys-290 by LYPLA1. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to inhibition of the recruitment of MAP1LC3A/LC3 to SQSTM1-positive structures. |
| Function | Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos. Also required to recruit ubiquitinated proteins to PML bodies in the nucleus. Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. Adapter that mediates the interaction between TRAF6 and CYLD. |
| Protein Name | Sequestosome-1Ebi3-Associated Protein Of 60 KdaEbiapP60Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 KdaUbiquitin-Binding Protein P62P62 |
| Database Links | Reactome: R-HSA-205043Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-5205685Reactome: R-HSA-8951664Reactome: R-HSA-9020702Reactome: R-HSA-9664873Reactome: R-HSA-9725370Reactome: R-HSA-9755511Reactome: R-HSA-9759194 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosomePreautophagosomal StructureCytoplasmCytosolNucleusPml BodyLate EndosomeLysosomeEndoplasmic ReticulumMyofibrilSarcomereIn Cardiac MuscleLocalizes To The Sarcomeric BandLocalizes To Cytoplasmic Membraneless Inclusion BodiesKnown As P62 BodiesContaining Polyubiquitinated Protein AggregatesIn Neurodegenerative DiseasesDetected In Lewy Bodies In Parkinson DiseaseNeurofibrillary Tangles In Alzheimer DiseaseAnd Htt Aggregates In Huntington DiseaseIn Protein Aggregate Diseases Of The LiverFound In Large Amounts In Mallory Bodies Of Alcoholic And Nonalcoholic SteatohepatitisHyaline Bodies In Hepatocellular CarcinomaAnd In Serpina1 AggregatesEnriched In Rosenthal Fibers Of Pilocytic AstrocytomaIn The CytoplasmObserved In Both Membrane-Free Ubiquitin-Containing Protein Aggregates (Sequestosomes) And Membrane-Surrounded AutophagosomesColocalizes With Trim13 In The Perinuclear Endoplasmic ReticulumCo-Localizes With Trim5 In Cytoplasmic BodiesWhen Nuclear Export Is Blocked By Treatment With Leptomycin BAccumulates In Pml Bodies |
| Alternative Antibody Names | Anti-Sequestosome-1 antibodyAnti-Ebi3-Associated Protein Of 60 Kda antibodyAnti-Ebiap antibodyAnti-P60 antibodyAnti-Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 Kda antibodyAnti-Ubiquitin-Binding Protein P62 antibodyAnti-P62 antibodyAnti-SQSTM1 antibodyAnti-ORCA antibodyAnti-OSIL antibody |
Information sourced from Uniprot.org
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