• Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-SOX2-Ser250/Ser251 antibody (STJ194747)

SKU:
STJ194747

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Transcription factor SOX-2-Ser250/Ser251 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOX2
Gene ID: 6657
Uniprot ID: SOX2_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Sox2 (phospho-Ser250 or Ser251)
Immunogen: Synthesized phosho peptide around human Sox2 (Ser250 and Ser251)
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Protein Name Transcription Factor Sox-2
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-3769402
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-8986944
Reactome: R-HSA-9754189
Cellular Localisation Nucleus Speckle
Cytoplasm
Nucleus
Acetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic Delocalization
Colocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)
Colocalizes With Sox6 In Speckles
Colocalizes With Caml In The Nucleus
Nuclear Import Is Facilitated By Xpo4
A Protein That Usually Acts As A Nuclear Export Signal Receptor
Alternative Antibody Names Anti-Transcription Factor Sox-2 antibody
Anti-SOX2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance