• Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody (STJ11101073) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Jurkat cells were treated by Anisomycin (25 ug/mL) at 37 °C for 20 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit polyclonal antibody (STJ11101073) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Jurkat cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-SMO-S611/615 antibody (STJ11101073)

SKU:
STJ11101073

£129.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMO
Gene ID: 6608
Uniprot ID: SMO_HUMAN
Specificity: A synthetic phosphorylated peptide around S611 & S615 of human SMO (NP_005622.1).
Immunogen Sequence: SADVS
Post Translational Modifications Phosphorylation by GRK kinases is required for interaction with protein kinase A catalytic subunit PRKACA.
Function G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.
Protein Name Protein Smoothened
Protein Gx
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5610787
Reactome: R-HSA-5620922
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Cilium
Cilium Localization Is Promoted By Shh And Is Required For Activity
Alternative Antibody Names Anti-Protein Smoothened antibody
Anti-Protein Gx antibody
Anti-SMO antibody
Anti-SMOH antibody

Information sourced from Uniprot.org

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