| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Mothers against decapentaplegic homolog 1-Ser465 (416-465 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD1 |
| Gene ID: |
4086 |
| Uniprot ID: |
SMAD1_HUMAN |
| Immunogen Region: |
416-465 aa |
| Specificity: |
Phospho-Smad1 (S465) Polyclonal Antibody detects endogenous levels of Smad1 protein only when phosphorylated at S465. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Smad1 around the phosphorylation site of Ser465 at the amino acid range 416-465 |
| Post Translational Modifications | Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Phosphorylation by ERK2 MAP kinase in response to EGF or HGF prevents SMAD1 nuclear accumulation and transcriptional activity in response to BMP. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Dephosphorylation is inhibited by association with EGR1. Phosphorylation by CDK8/9 creates binding sites for YAP1, and subsequent phosphorylation by GSK3 switches off YAP1 binding and adds binding sites for SMURF1. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Phospho-SMAD1 is ubiquitinated by CHIP leading to disruption of the SMAD1-SMAD4 complex. |
| Function | Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import. |
| Protein Name | Mothers Against Decapentaplegic Homolog 1Mad Homolog 1Mothers Against Dpp Homolog 1Jv4-1Mad-Related Protein 1Smad Family Member 1Smad 1Smad1Hsmad1Transforming Growth Factor-Beta-Signaling Protein 1Bsp-1 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-5689880Reactome: R-HSA-8941326Reactome: R-HSA-9733709 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4Co-Localizes With Lemd3 At The Nucleus Inner MembraneExported From The Nucleus To The Cytoplasm When Dephosphorylated |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 1 antibodyAnti-Mad Homolog 1 antibodyAnti-Mothers Against Dpp Homolog 1 antibodyAnti-Jv4-1 antibodyAnti-Mad-Related Protein 1 antibodyAnti-Smad Family Member 1 antibodyAnti-Smad 1 antibodyAnti-Smad1 antibodyAnti-Hsmad1 antibodyAnti-Transforming Growth Factor-Beta-Signaling Protein 1 antibodyAnti-Bsp-1 antibodyAnti-SMAD1 antibodyAnti-BSP1 antibodyAnti-MADH1 antibodyAnti-MADR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
