• Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5', using SHP-1 (Phospho-Tyr536) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SHP-1 (Phospho-Tyr536) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of RAW264.7+EGF cells using Phospho-SH-PTP1 (Y536) Polyclonal Antibody

Anti-Phospho-PTPN6-Tyr536 antibody (502-551 aa) (STJ90740)

SKU:
STJ90740

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein phosphatase non-receptor type 6-Tyr536 (502-551 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN6
Gene ID: 5777
Uniprot ID: PTN6_HUMAN
Immunogen Region: 502-551 aa
Specificity: Phospho-SH-PTP1 (Y536) Polyclonal Antibody detects endogenous levels of SH-PTP1 protein only when phosphorylated at Y536.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SHP-1 around the phosphorylation site of Tyr536 at the amino acid range 502-551
Post Translational Modifications Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity.
Function Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 6
Hematopoietic Cell Protein-Tyrosine Phosphatase
Protein-Tyrosine Phosphatase 1c
Ptp-1c
Protein-Tyrosine Phosphatase Shp-1
Sh-Ptp1
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1433559
Reactome: R-HSA-201556
Reactome: R-HSA-210990
Reactome: R-HSA-388841
Reactome: R-HSA-389948
Reactome: R-HSA-391160
Reactome: R-HSA-432142
Reactome: R-HSA-512988
Reactome: R-HSA-5690714
Reactome: R-HSA-6798695
Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-9008059
Reactome: R-HSA-909733
Reactome: R-HSA-912526
Reactome: R-HSA-912694
Reactome: R-HSA-9705671
Reactome: R-HSA-982772
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Nucleus
In Neurons
Translocates Into The Nucleus After Treatment With Angiotensin Ii
Shuttles Between The Cytoplasm And Nucleus Via Its Association With Pdpk1
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 6 antibody
Anti-Hematopoietic Cell Protein-Tyrosine Phosphatase antibody
Anti-Protein-Tyrosine Phosphatase 1c antibody
Anti-Ptp-1c antibody
Anti-Protein-Tyrosine Phosphatase Shp-1 antibody
Anti-Sh-Ptp1 antibody
Anti-PTPN6 antibody
Anti-HCP antibody
Anti-PTP1C antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance