• Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SGK3-Thr320 antibody (STJ196416)

SKU:
STJ196416

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase Sgk3-Thr320 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGK3
Gene ID: 100533105/23678
Uniprot ID: SGK3_HUMAN
Specificity: This antibody detects endogenous levels of Human SGK3 (phospho-Thr320)
Immunogen: Synthesized phosho peptide around human SGK3 (Thr320)
Post Translational Modifications Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Function Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Protein Name Serine/Threonine-Protein Kinase Sgk3
Cytokine-Independent Survival Kinase
Serum/Glucocorticoid-Regulated Kinase 3
Serum/Glucocorticoid-Regulated Kinase-Like
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cytoplasmic Vesicle
Early Endosome
Recycling Endosome
Endosomal Localization Is A Prerequisite For Complete Kinase Activity
It Is Essential For Its Colocalization With The Kinase Responsible For Phosphorylating Ser-486 Thus Allowing Pdpk1 Phosphorylation Of Thr-320 Resulting In Complete Activation Of Sgk3
Localized In Vesicle-Like Structures And In The Early Endosome
Colocalizes With Slc9a3/Nhe3 In The Recycling Endosomes
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Sgk3 antibody
Anti-Cytokine-Independent Survival Kinase antibody
Anti-Serum/Glucocorticoid-Regulated Kinase 3 antibody
Anti-Serum/Glucocorticoid-Regulated Kinase-Like antibody
Anti-SGK3 antibody
Anti-CISK antibody
Anti-SGKL antibody

Information sourced from Uniprot.org

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