• Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Syndecan4 (Phospho-Ser179) Antibody
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SDC4-Ser179 antibody (145-194 aa) (STJ91310)

SKU:
STJ91310

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Syndecan-4-Ser179 (145-194 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDC4
Gene ID: 6385
Uniprot ID: SDC4_HUMAN
Immunogen Region: 145-194 aa
Specificity: Phospho-Syndecan-4 (S179) Polyclonal Antibody detects endogenous levels of Syndecan-4 protein only when phosphorylated at S179.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Syndecan4 around the phosphorylation site of Ser179 at the amino acid range 145-194
Post Translational Modifications Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. O-glycosylated.contains both chondroitin sulfate and heparan sulfate. Ser-39, Ser-61 and Ser-63 can all be modified by either chondroitin sulfate or heparan sulfate, and the protein exists in forms that contain only chondroitin sulfate, only heparan sulfate and both chondroitin sulfate and heparan sulfate.
Function Cell surface proteoglycan which regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Protein Name Syndecan-4
Synd4
Amphiglycan
Ryudocan Core Protein
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-202733
Reactome: R-HSA-3000170
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Secreted
Shedding Of The Ectodomain Produces A Soluble Form
Isoform 2: Secreted
Alternative Antibody Names Anti-Syndecan-4 antibody
Anti-Synd4 antibody
Anti-Amphiglycan antibody
Anti-Ryudocan Core Protein antibody
Anti-SDC4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance