| Host: | Rabbit |
| Applications: | WB/IF-P/IHC-P/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM13700 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS containing 50% Glycerol and 0.05% BSA, preserved with proclin300 or Sodium Azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB, 1:1000-1:4000IF-P, 1:50-1:200IHC-P, 1:500-1:1000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPA2 |
| Gene ID: | 6118 |
| Uniprot ID: | RFA2_HUMAN |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence: | Human |
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. |
| Protein Name | Replication Protein A 32 Kda SubunitRp-A P32Replication Factor A Protein 2Rf-A Protein 2Replication Protein A 34 Kda SubunitRp-A P34 |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-174437Reactome: R-HSA-176187Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-5696395Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6783310Reactome: R-HSA-6804756Reactome: R-HSA-68962Reactome: R-HSA-69166Reactome: R-HSA-69473Reactome: R-HSA-912446Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusPml BodyRedistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner |
| Alternative Antibody Names | Anti-Replication Protein A 32 Kda Subunit antibodyAnti-Rp-A P32 antibodyAnti-Replication Factor A Protein 2 antibodyAnti-Rf-A Protein 2 antibodyAnti-Replication Protein A 34 Kda Subunit antibodyAnti-Rp-A P34 antibodyAnti-RPA2 antibodyAnti-REPA2 antibodyAnti-RPA32 antibodyAnti-RPA34 antibody |
Information sourced from Uniprot.org
