• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RICTOR-Thr1135 antibody (STJ196401)

SKU:
STJ196401

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Rapamycin-insensitive companion of mTOR-Thr1135 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RICTOR
Gene ID: 253260
Uniprot ID: RICTR_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rictor (phospho-Thr1135)
Immunogen: Synthesized phosho peptide around human Rictor (Thr1135)
Function Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Protein Name Rapamycin-Insensitive Companion Of Mtor
Avo3 Homolog
Havo3
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-389357
Reactome: R-HSA-5218920
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Alternative Antibody Names Anti-Rapamycin-Insensitive Companion Of Mtor antibody
Anti-Avo3 Homolog antibody
Anti-Havo3 antibody
Anti-RICTOR antibody
Anti-KIAA1999 antibody

Information sourced from Uniprot.org

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