| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Rhodopsin-Ser334 (299-348 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RHO |
| Gene ID: |
6010 |
| Uniprot ID: |
OPSD_HUMAN |
| Immunogen Region: |
299-348 aa |
| Specificity: |
Phospho-Rhodopsin (S334) Polyclonal Antibody detects endogenous levels of Rhodopsin protein only when phosphorylated at S334. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Rhodopsin around the phosphorylation site of Ser334 at the amino acid range 299-348 |
| Post Translational Modifications | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. After activation by light, phosphorylated by GRK1 (in vitro). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal. |
| Function | Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling. |
| Protein Name | RhodopsinOpsin-2 |
| Database Links | Reactome: R-HSA-2453902Reactome: R-HSA-2485179Reactome: R-HSA-2514859Reactome: R-HSA-418594Reactome: R-HSA-419771Reactome: R-HSA-5620916 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumPhotoreceptor Outer SegmentSynthesized In The Inner Segment (Is) Of Rod Photoreceptor Cells Before Vectorial Transport To Disk Membranes In The Rod Outer Segment (Os) Photosensory Cilia |
| Alternative Antibody Names | Anti-Rhodopsin antibodyAnti-Opsin-2 antibodyAnti-RHO antibodyAnti-OPN2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
