| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Phospho-Rb2 p130-Ser952 for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R02-5D6 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | RBL2 |
| Gene ID: | 5934 |
| Uniprot ID: | RBL2_HUMAN |
| Immunogen: | A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130 |
| Post Translational Modifications | During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. |
| Protein Name | Retinoblastoma-Like Protein 2130 Kda Retinoblastoma-Associated ProteinP130Retinoblastoma-Related Protein 2Rbr-2Prb2 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-6804114Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656Reactome: R-HSA-9617828 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Retinoblastoma-Like Protein 2 antibodyAnti-130 Kda Retinoblastoma-Associated Protein antibodyAnti-P130 antibodyAnti-Retinoblastoma-Related Protein 2 antibodyAnti-Rbr-2 antibodyAnti-Prb2 antibodyAnti-RBL2 antibodyAnti-RB2 antibody |
Information sourced from Uniprot.org
