Anti-Phospho-RAPGEF1-Tyr504 antibody (470-519 aa) (STJ90537)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RapGEF1 around the phosphorylation site of Tyr504 at the amino acid range 470-519
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Rap guanine nucleotide exchange factor 1-Tyr504 (470-519 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAPGEF1 |
| Gene ID | 2889 |
| Uniprot ID | RPGF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RapGEF1 around the phosphorylation site of Tyr504 at the amino acid range 470-519 |
| Immunogen Region | 470-519 aa |
| Specificity | Phospho-C3G (Y504) Polyclonal Antibody detects endogenous levels of C3G protein only when phosphorylated at Y504. |
Additional Info
| Post Translational Modifications | Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor. |
| Function | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth. |
| Protein Name | Rap Guanine Nucleotide Exchange Factor 1Crk Sh3-Binding GnrpGuanine Nucleotide-Releasing Factor 2Protein C3g |
| Database Links | Reactome: R-HSA-170968Reactome: R-HSA-186763Reactome: R-HSA-8875555Reactome: R-HSA-9013423Reactome: R-HSA-9027284Reactome: R-HSA-912631 |
| Cellular Localisation | Early Endosome |
| Alternative Antibody Names | Anti-Rap Guanine Nucleotide Exchange Factor 1 antibodyAnti-Crk Sh3-Binding Gnrp antibodyAnti-Guanine Nucleotide-Releasing Factor 2 antibodyAnti-Protein C3g antibodyAnti-RAPGEF1 antibodyAnti-GRF2 antibody |
Information sourced from Uniprot.org