Western blot analysis of lysates from HeLa cells, using Phospho-Paxillin-Y118 Rabbit pAb (STJ22361) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PXN-Y118 antibody (STJ22361)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ22361
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General Information

Short DescriptionRabbit polyclonal Phospho-PXN-Y118 antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPXN
Gene ID5829
Uniprot IDPAXI_HUMAN
Immunogen SequenceHVYSF
SpecificityA synthetic phosphorylated peptide around Y118 of human Paxillin (NP_001074324.1).

Additional Info

Post Translational Modifications Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility. Phosphorylation at Ser-272 promotes focal adhesion disassembly during cell migration.
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Protein Name Paxillin
Database Links Reactome: R-HSA-180292
Reactome: R-HSA-4420097
Reactome: R-HSA-445355
Reactome: R-HSA-446343
Reactome: R-HSA-446388
Reactome: R-HSA-8849471
Reactome: R-HSA-9926550
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Focal Adhesion
Cell Cortex
Colocalizes With Integrins At The Cell Periphery
Colocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Paxillin antibody
Anti-PXN antibody

Information sourced from Uniprot.org

Citations

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