Anti-Phospho-PTPN1-Ser50 antibody (16-65 aa) (STJ90882)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PTP1B around the phosphorylation site of Ser50 at the amino acid range 16-65
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Tyrosine-protein phosphatase non-receptor type 1-Ser50 (16-65 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PTPN1 |
| Gene ID | 5770 |
| Uniprot ID | PTN1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PTP1B around the phosphorylation site of Ser50 at the amino acid range 16-65 |
| Immunogen Region | 16-65 aa |
| Specificity | Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50. |
Additional Info
| Post Translational Modifications | Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity. |
| Function | Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 1Protein-Tyrosine Phosphatase 1bPtp-1b |
| Database Links | Reactome: R-HSA-354192Reactome: R-HSA-6807004Reactome: R-HSA-77387Reactome: R-HSA-877312Reactome: R-HSA-8849472Reactome: R-HSA-9022699Reactome: R-HSA-912694Reactome: R-HSA-982772Reactome: R-HSA-9860927 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideInteracts With Epha3 At The Cell Membrane |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibodyAnti-Protein-Tyrosine Phosphatase 1b antibodyAnti-Ptp-1b antibodyAnti-PTPN1 antibodyAnti-PTP1B antibody |
Information sourced from Uniprot.org