• Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr410) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using PKC zeta (Phospho-Thr410) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of 823 cells using Phospho-PKC Zeta (T410) Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using Phospho-PKC Zeta (T410) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCZ-Thr410 antibody (376-425 aa) (STJ90389)

SKU:
STJ90389

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein kinase C zeta type-Thr410 (376-425 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKCZ
Gene ID: 5590
Uniprot ID: KPCZ_HUMAN
Immunogen Region: 376-425 aa
Specificity: Phospho-PKC Zeta (T410) Polyclonal Antibody detects endogenous levels of PKC Zeta protein only when phosphorylated at T410.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PKC zeta around the phosphorylation site of Thr410 at the amino acid range 376-425
Post Translational Modifications CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1.phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.
Function Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro. Isoform 2: Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
Protein Name Protein Kinase C Zeta Type
Npkc-Zeta
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-2173791
Reactome: R-HSA-5218921
Reactome: R-HSA-5668599
Reactome: R-HSA-9634635
Cellular Localisation Cytoplasm
Endosome
Cell Junction
Membrane
Peripheral Membrane Protein
In The Retina
Localizes In The Terminals Of The Rod Bipolar Cells
Associates With Endosomes
Presence Of Krit1
Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction
Colocalizes With Vamp2 And Wdfy2 In Intracellular Vesicles
Transiently Translocates To The Membrane Of Ca1 Hippocampal Cells In Response To The Induction Of Long Term Potentiation
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Protein Kinase C Zeta Type antibody
Anti-Npkc-Zeta antibody
Anti-PRKCZ antibody
Anti-PKC2 antibody

Information sourced from Uniprot.org

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