Anti-Phospho-PRAG1-Tyr413 antibody (STJ90396)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phospho-peptide around the phosphorylation site of human Pragmin (phospho Tyr413)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Inactive tyrosine-protein kinase PRAG1-Tyr413 for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRAG1 |
| Gene ID | 157285 |
| Uniprot ID | PRAG1_HUMAN |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Pragmin (phospho Tyr413) |
| Specificity | Phospho-Pragmin (Y413) Polyclonal Antibody detects endogenous levels of Pragmin protein only when phosphorylated at Y413. |
Additional Info
| Function | Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth. Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism. Acts as a critical coactivator of Notch signaling. |
| Protein Name | Inactive Tyrosine-Protein Kinase Prag1Peak1-Related Kinase-Activating Pseudokinase 1PragminSugen Kinase 223Sgk223 |
| Database Links | Reactome: R-HSA-9696270 |
| Cellular Localisation | CytoplasmCell JunctionFocal AdhesionNucleusColocalized With Notch1 In The Nucleus |
| Alternative Antibody Names | Anti-Inactive Tyrosine-Protein Kinase Prag1 antibodyAnti-Peak1-Related Kinase-Activating Pseudokinase 1 antibodyAnti-Pragmin antibodyAnti-Sugen Kinase 223 antibodyAnti-Sgk223 antibodyAnti-PRAG1 antibodyAnti-SGK223 antibody |
Information sourced from Uniprot.org