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Anti-Phospho-PPP2CA-Tyr307 antibody (260-309 aa) (STJ91049)

Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Western blot analysis of SKOV3 using p-PP2A-C Alpha (Y307) antibody. Antibody was diluted at 1:1000
Immunofluorescence analysis of Hela cell. 1, PP2A-C Alpha (phospho Tyr307) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-PPP2CA-Tyr307 antibody (260-309 aa) (STJ91049)

SKU:

STJ91049

£48.00 - £252.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IP/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform-Tyr307 (260-309 aa) is suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and ELISA research

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PPP2CA
Gene ID:	5515
Uniprot ID:	PP2AA_HUMAN
Immunogen Region:	260-309 aa
Specificity:	Phospho-PP2A-C Alpha (Y307) Polyclonal Antibody detects endogenous levels of PP2A-C Alpha protein only when phosphorylated at Y307.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PP2A-alpha around the phosphorylation site of Tyr307 at the amino acid range 260-309

Function	Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.
Protein Name	Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform Pp2a-Alpha Replication Protein C Rp-C
Database Links	Reactome: R-HSA-113501 Reactome: R-HSA-1295596 Reactome: R-HSA-141444 Reactome: R-HSA-163685 Reactome: R-HSA-163767 Reactome: R-HSA-180024 Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2465910 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2995383 Reactome: R-HSA-389513 Reactome: R-HSA-432142 Reactome: R-HSA-4641262 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5467337 Reactome: R-HSA-5467340 Reactome: R-HSA-5467348 Reactome: R-HSA-5663220 Reactome: R-HSA-5673000 Reactome: R-HSA-5675221 Reactome: R-HSA-6804757 Reactome: R-HSA-6811558 Reactome: R-HSA-68877 Reactome: R-HSA-69231 Reactome: R-HSA-69273 Reactome: R-HSA-9634600 Reactome: R-HSA-9648025 Reactome: R-HSA-975957 Reactome: R-HSA-9833482
Cellular Localisation	Cytoplasm Nucleus Chromosome Centromere Cytoskeleton Spindle Pole In Prometaphase Cells But Not In Anaphase Cells Localizes At Centromeres During Mitosis Also Found At Spindle Poles Centromeric Localization Requires The Presence Of Sgo2
Alternative Antibody Names	Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody Anti-Pp2a-Alpha antibody Anti-Replication Protein C antibody Anti-Rp-C antibody Anti-PPP2CA antibody

Information sourced from Uniprot.org

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