• Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).
  • Effects of striatal slice treatment with NMDA (25 Mu M, 5 min) , dopamine (DA, 10 Mu M, 15 min) , or both on PKA-dependent phosphorylation of Ser845 GluR1 (top) and Thr34 DARPP-32 (bottom). Image from publication CC-BY-4. 0. PMID: 35835216

Anti-Phospho-PPP1R1B-Thr34 antibody (STJA0003607)

SKU:
STJA0003607-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DARPP-32-Thr34 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ppp1r1b
Gene ID: 360616
Uniprot ID: PPR1B_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH.
Post Translational Modifications Phosphorylation of Thr-34 is required for activity. Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Function Inhibitor of protein-phosphatase 1.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1b
Darpp-32
Dopamine- And Camp-Regulated Neuronal Phosphoprotein
Database Links Reactome: R-RNO-180024
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 1b antibody
Anti-Darpp-32 antibody
Anti-Dopamine- And Camp-Regulated Neuronal Phosphoprotein antibody
Anti-Ppp1r1b antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance