• Western blot analysis of extracts of HeLa cells, using Phospho-PLCB3-S1105 antibody (STJ11100978) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3) -S1105 Rabbit polyclonal antibody (STJ11100978) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PLCB3-S1105 antibody (STJ11100978)

SKU:
STJ11100978

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PLCB3-S1105 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCB3
Gene ID: 5331
Uniprot ID: PLCB3_HUMAN
Immunogen: A synthetic phosphorylated peptide around S1105 of human PLC beta 3 (PLCB3) (NP_000923.1).
Immunogen Sequence: HNSIS
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-3
Phosphoinositide Phospholipase C-Beta-3
Phospholipase C-Beta-3
Plc-Beta-3
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-1855204
Reactome: R-HSA-399997
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-418217
Reactome: R-HSA-434316
Reactome: R-HSA-500657
Cellular Localisation Cytoplasm
Membrane
Nucleus
And Particulate Fractions
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-3 antibody
Anti-Phosphoinositide Phospholipase C-Beta-3 antibody
Anti-Phospholipase C-Beta-3 antibody
Anti-Plc-Beta-3 antibody
Anti-PLCB3 antibody

Information sourced from Uniprot.org

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