• Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKR (Phospho-Thr258) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKR (Phospho-Thr258) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EIF2AK2-Thr258 antibody (226-275 aa) (STJ90956)

SKU:
STJ90956

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Interferon-induced, double-stranded RNA-activated protein kinase-Thr258 (226-275 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2AK2
Gene ID: 5610
Uniprot ID: E2AK2_HUMAN
Immunogen Region: 226-275 aa
Specificity: Phospho-PKR (T258) Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at T258.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PKR around the phosphorylation site of Thr258 at the amino acid range 226-275
Post Translational Modifications Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation.
Function IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteasomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding pro-inflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Protein Name Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase
Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2
Eif-2a Protein Kinase 2
Interferon-Inducible Rna-Dependent Protein Kinase
P1/Eif-2a Protein Kinase
Protein Kinase Rna-Activated
Pkr
Protein Kinase R
Tyrosine-Protein Kinase Eif2ak2
P68 Kinase
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-169131
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Nucleus
Perinuclear Region
Nuclear Localization Is Elevated In Acute Leukemia
Myelodysplastic Syndrome (Mds)
Melanoma
Breast
Colon
Prostate And Lung Cancer Patient Samples Or Cell Lines As Well As Neurocytes From Advanced Creutzfeldt-Jakob Disease Patients
Alternative Antibody Names Anti-Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase antibody
Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2 antibody
Anti-Eif-2a Protein Kinase 2 antibody
Anti-Interferon-Inducible Rna-Dependent Protein Kinase antibody
Anti-P1/Eif-2a Protein Kinase antibody
Anti-Protein Kinase Rna-Activated antibody
Anti-Pkr antibody
Anti-Protein Kinase R antibody
Anti-Tyrosine-Protein Kinase Eif2ak2 antibody
Anti-P68 Kinase antibody
Anti-EIF2AK2 antibody
Anti-PKR antibody
Anti-PRKR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance