• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000
  • Western Blot analysis of 1 NIH/3T3 treated with LPS, 2 NIH/3T3, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PKN1-Thr816 antibody (STJ196390)

SKU:
STJ196390

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase N1-Thr816 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PKN1
Gene ID: 5585
Uniprot ID: PKN1_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rat PRK1 (phospho-Thr774) or PRK2 (phospho-Thr816)
Immunogen: Synthesized phosho peptide around human PRK1 (Thr774) and PRK2 (Thr816)
Post Translational Modifications Autophosphorylated.preferably on serine. Phosphorylated during mitosis. Activated by limited proteolysis with trypsin. (Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
Function PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Protein Name Serine/Threonine-Protein Kinase N1
Protease-Activated Kinase 1
Pak-1
Protein Kinase C-Like 1
Protein Kinase C-Like Pkn
Protein Kinase Pkn-Alpha
Protein-Kinase C-Related Kinase 1
Serine-Threonine Protein Kinase N
Database Links Reactome: R-HSA-5625740
Reactome: R-HSA-5625886
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Nucleus
Endosome
Cell Membrane
Peripheral Membrane Protein
Cleavage Furrow
Midbody
Associates With Chromatin In A Ligand-Dependent Manner
Localization To Endosomes Is Mediated Via Its Interaction With Rhob
Association To The Cell Membrane Is Dependent On Ser-377 Phosphorylation
Accumulates During Telophase At The Cleavage Furrow And Finally Concentrates Around The Midbody In Cytokinesis
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase N1 antibody
Anti-Protease-Activated Kinase 1 antibody
Anti-Pak-1 antibody
Anti-Protein Kinase C-Like 1 antibody
Anti-Protein Kinase C-Like Pkn antibody
Anti-Protein Kinase Pkn-Alpha antibody
Anti-Protein-Kinase C-Related Kinase 1 antibody
Anti-Serine-Threonine Protein Kinase N antibody
Anti-PKN1 antibody
Anti-PAK1 antibody
Anti-PKN antibody
Anti-PRK1 antibody
Anti-PRKCL1 antibody

Information sourced from Uniprot.org

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